High-Molecular-Weight Kininogen from Horse Plasma
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چکیده
منابع مشابه
Evidence that T-kinin does not mediate paw swelling induced by carrageenin: studies with plasma high molecular weight kininogen and low molecular weight kininogen deficient rats.
Paw swelling induced by carrageenin was compared in Brown Norway Kitasato rats (normal) and Brown Norway Katholiek rats, deficient in HMW and LMW kininogens. The increase in the paw volume of the former after the carrageenin injection was about 50-70% of the initial volume, whereas that of the latter was about 10%. Pretreatment with captopril, a kininase inhibitor, enhanced the edema significan...
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To study the digestion pattern of human high-molecular weight (mol wt) kininogen (HMWK) in plasma during contact activation we have prepared monoclonal antibodies (MoAbs) to the light-chain (LC) and the heavy-chain moiety of HMWK. One MoAb from each set was purified, and neither MoAb inhibited the clotting activity of HMWK. In enzyme-linked immunosorbent assay and immunoblotting experiments nei...
متن کاملAn essential role of high-molecular-weight kininogen in endotoxemia
In this study, we show that mice lacking high-molecular-weight kininogen (HK) were resistant to lipopolysaccharide (LPS)-induced mortality and had significantly reduced circulating LPS levels. Replenishment of HK-deficient mice with human HK recovered the LPS levels and rendered the mice susceptible to LPS-induced mortality. Binding of HK to LPS occurred through the O-polysaccharide/core oligos...
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An anti-cell adhesion globulin was purified from human plasma by heparin-affinity chromatography. The purified globulin inhibited spreading of osteosarcoma and melanoma cells on vitronectin, and of endothelial cells, platelets, and mononuclear blood cells on vitronectin or fibrinogen. It did not inhibit cell spreading on fibronectin. The protein had the strongest antiadhesive effect when preads...
متن کاملHigh molecular weight kininogen binds to unstimulated platelets.
Studies were performed to determine if the unstimulated platelet membrane has a site for high molecular weight kininogen (HMWK) binding. 125I-HMWK bound to unstimulated platelets. Zn++ was required for 125I-HMWK binding to unstimulated platelets and binding was maximal at 50 microM Zn++. Neither Mg++ nor Ca++ substituted for Zn++ in supporting 125I-HMWK binding to unstimulated platelets, and ne...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2005
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1981.tb06221.x